Modern Water RaPID Assay PCBs 100 Tube Kit - A00134

A00134 RaPID Assay PCBs 100 Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Results available in approximately 60 minutes
• Training available
• Magnetic particle immunoassay
• EPA SW-846 method #4020

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80+ samples)

Assay range

• Soil: 0.5 ppm to 10 ppm total PCB's as Aroclor 1254
• Water: 0.25 to 5.0 ppb total PCB’s as Aroclor 1254
• Wipes: 5 to 100μg per wipe total PCB’s as Aroclor 1254
• Range can be extended with additional dilutions

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions with methanol.
• Water samples must be diluted one part sample to one part methanol to prevent adsorptive loss.

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes

Specificity

The table below shows compounds at the method detection limit(MDL) which isthe lowest concentration of the compound that can be picked up in the assay. The limit of quantification (LOQ)- an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Basic Test Procedure

• Add calibrators and sample, enzyme conjugate, and antibody coupled magnetic particles to a test tube. Vortex.
• Incubate for 15 minutes
• Using the RaPID magnetic separator, decant and wash two times
• Add colour solution and incubate for 20 minutes
• Stop the reaction and read colour at 450 nm
• Quantitive results and QC parameters are calculated and printed automatically using the RPA spectrophotometer

Test kit components

• Antibody coated magnetic particles for analysis of 100 test tubes
• Zero standard, wash, enzyme conjugate, colour development and stop reagents
• Standards for 0.25, 1.00 and 5.0 ppb as Aroclor 1254
• Kit control as 3.0 ppb as Aroclor 1254
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• PCB Assay 100 tube kit (A00134)
• Sample extraction kit(for soil samples only) (A00137EB)
• Sample extraction kit(for wipe samples only) (A00137WB)
• PCB sample diluent 100mL (A00136)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-I1 RaPID analyser (6000081), Printer (6000082), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips(12.5 mL) (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen