Modern Water 2, 4-D 100 Tube Kit - A00082

A00082 2, 4-D 100 Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Test up to 50 samples at one time
• Results available in approximately 60 minutes
• Training available
• Magnetic particle immunoassay
• EPA SW-846 method #4015

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80+ samples)

Assay range

• Soil: 0.15 ppm to 7.5 ppm as 2, 4-D
• Water: 1.0 to 50.0 ppb as 2, 4-D
• Range can be extended with additional dilutions

Sample preparation

• Soil samples require prior extraction as indicated in the application procedure

Sampling time

• A 16 hour extraction time is recommended for soil Method 4015

Specificity

The 2, 4D RaPID Assay immunoassay test does not differentiate between 2,4-D and other chlorophenoxy acids. The table below shows compounds at the limit of quantitation (LOQ) -an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the color produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Basic Test Procedure

• Add standards, prepared sample, enzyme conjugate, and antibody coupled magnetic particles.Vortex.
• Incubate for 30 minutes
• Using the magnetic separator, decant and wash
• Add colour solution, incubate 20 minutes
• Stop the reaction and read colour at 450 nm
• Quantitative results and QC parameters are calculated and printed automatically using the RPA-1I spectrophotometer

Test kit components

• Antibody coated magnetic particles for analysis of 100 test tubes
• Zero standard, wash, enzyme conjugate, colour development and stop reagents
• Standards for 1, 10 and 50 ppb as 2, 4-D
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use for up to 8 hours
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• 2, 4-D 100 tube kit (A00082)
• 2, 4-D sample diluent (A00079)

Required test equipment

• RaPID Assay accessory kit (6050100 (P)/6997010 (R)) which contains: RPAI1 analyser (6000081), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips (12.5 mL) (A00013)
• Adjustable pipet tips

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen