Modern Water RaPID Assay Pentachlorophenol (PCP) 100 Tube Test Kit - A00111

A00111 RaPID Assay Pentachlorophenol (PCP) 100 Tube Test Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Tests up to 50 samples at once
• Results available in approximately 60 minutes
• Magnetic particle immunoassay
• Training available
• EPA SW-846 method #4010

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80 samples)

Assay range

• Soil: 0.1 ppm to 10 ppm as pentachlorophenol
• Water: 0.1 to 10.0 ppb as pentachlorophenol
• Other applications are also available
• Range can be extended with additional dilutions

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions with alkaline methanol.

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes.

Specificity

The PCP RaPID Assay® immunoassay test does not differentiate between PCP and other organochlorines. The table below shows compounds at the limit of quantitation (LOQ)- an approximate concentration required to yield a positive result at the lowest standard. The IC50 isthe concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Basic Test Procedure

• Add prepared sample, enzyme conjugate, and antibody coupled magnetic particles.Vortex
• Incubate for 30 minutes
• Separate using the RaPID magnetic separator. decant and wash
• Add colour solution and incubate for 20 minutes
• Stop the reaction and read colour at 450 nm
• Quantitive results and QC parameters are calculated and printed automatically using the RPA-1I spectrophotometer

Test kit components

• Antibody coated magnetic particles for analysis of 100 test tubes
• Zero standard, wash, enzyme conjugate, colour development and stop reagents
• Standards for 0.1, 2 and 10 ppb as PCP
• Kit control as 1.0 ppb as PCP
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) for up to eight hours
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight
• Portable spectrophotometer battery should be charged prior to use

Required test materials

• PCP 100 tube test kit (A00111)
• Sample extraction kit (A00128EA)
• PCP sample diluent(as needed) (A00113)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-I1 RaPID analyser (6000111), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen